The present invention relates to method of cultivating rat mesenchymal stem cells to which tissue transglutaminase gene (variant 2) is transferred by lentiviral transduction, on three dimensional poly (1, 4-butylene succinate ) / poly (L-lactic acid) tissue scaffolds. The objective of the present invention is to cultivate the cells to which gene delivery is performed using lentivirus, on polymeric tissue scaffolds, and then to use them in cartilage tissue defects and tissue regeneration.