This invention introduces a novel medium-based method for differentiating dental mesenchymal stem cells into dopaminergic neurons. Using a two-step neurogenic induction protocol, cell cycle arrest is achieved, leading to rapid and efficient neuronal differentiation. The method ensures terminal neuronal transformation without toxicity, confirmed through morphological, genetic, and functional analyses. This technology offers a valuable platform for regenerative medicine in treating neurodegenerative diseases such as Parkinson's and Alzheimer's, and for developing patient-specific disease models for drug discovery and neuroscience research.